磷酸化活化T细胞核因子5蛋白抗体
规格：1mg/1ml
英文名： phospho-NFAT5 (Ser1197)
别名： NFAT5 (phospho S1197); p-NFAT5(phospho S1197); Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of
分子量： 166kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
细胞定位：细胞核
磷酸化活化T细胞核因子5蛋白抗体产品介绍：background: Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating 磷酸化活化T细胞核因子5蛋白抗体NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus. Function: Plays a role in the inducible expression of genes.Regulates hypertonicity-induced cellular accumulation of osmolytes. Subunit: Homodimer when bound to DNA, completely encircles its DNAtarget. Does not bind with Fos and Jun transcription factors. Subcellular Location: Nucleus. Tissue Specificity: Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Post-translational modifications: Phosphorylated at Thr-135 by CDK5 in response to osmoticstress; this phosphorylation mediates its rapid 磷酸化活化T细胞核因子5蛋白抗体nuclearlocalization. Similarity: Contains 1 RHD (Rel-like) domain. Database links: Entrez Gene: 10725 Human Entrez Gene: 54446 Mouse Entrez Gene: 307820 Rat Omim: 604708 Human SwissProt: O94916 Human SwissProt: Q9WV30 Mouse Unigene: 371987 Human Unigene: 390057 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化活化T细胞核因子5蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：心血管 转录调节因子 **细胞 t-**细胞 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid