磷酸化丝裂原活化蛋白激酶3抗体
规格：1mg/1ml
英文名： phospho-MAPK3(Tyr204)
别名： ERK1 (phospho Y204); p-ERK1 (phospho Y204); MAPK3(phospho Y204); ERK/MAPK(phospho T202/Y204); p44/42 MAP Kinase(Phospho-Thr202); ERK 1; ERK; ERK-1; ERK1; Extracellular Signal Regulated Kinase 1; Extra
分子量： 43kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from rat M
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
细胞定位：细胞浆
磷酸化丝裂原活化蛋白激酶3抗体产品介绍：background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. Function: Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. MAPK1/ERK2and MAPK3/ERK1 are the 2 MAPKs which play an important role in theMAPK/ERK cascade. They participate also in a signaling cascadeinitiated by activated KIT and KITLG/SCF. Depending on the cellularcontext, the MAPK/ERK cascade mediates diverse biological functionssuch as cell growth, adhesion, survival and differentiation throughthe regulation of transcription, translation, cytoskeletalrearrangements. The MAPK/ERK cascade plays also a role ininitiation and regulation of meiosis, mitosis, and postmitoticfunctions in differentiated cells by phosphorylating a number oftranscription factors. About 160 substrates have already beendiscovered for ERKs. Many of these substrates are localized in thenucleus, and seem to participate in the regulation of transcriptionupon stimulation. However, other substrates are found in thecytosol as well as in other cellular organelles, and those areresponsible for processes such as translation, mitosis andapoptosis. Moreover, the MAPK/ERK cascade is also involved in theregulation of the endosomal dynamics, including lysosome processingand endosome cycling through the perinuclear recycling compartment(PNRC); as well as in the fragmentation of the磷酸化丝裂原活化蛋白激酶3抗体Golgi apparatusduring mitosis. The substrates include transcription factors (suchas ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements(such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1),regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3,MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and avariety of other signaling-related molecules (like ARHGEF2, FRS2 orGRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2,RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2,RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases(such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates whichenable the propagation the MAPK/ERK signal to additional cytosolicand nuclear targets, thereby extending the specificity of thecascade. Subunit: Binds both upstream activators and downstream substratesin multimolecular complexes. Found in a complex with at least BRAF,HRAS1, 磷酸化丝裂原活化蛋白激酶3抗体MAP2K1/MEK1, MAPK3 and RGS14. Interacts with ADAM15, ARRB2,CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1,NISCH, PEA15, SGK1 and MKNK2 (By similarity). MKNK2 isoform 1binding prevents from dephosphorylation and inactivation. Interactswith TPR (By similarity). Subcellular Location: Cytoplasm (By similarity). Nucleus.Note=Autophosphorylation at Thr-207 promotes nuclear localization(By similarity). PEA15-binding redirects the biological outcome ofMAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (Bysimilarity). Isoform 2: Nucleus. Tissue Specificity: Highest levels within the nervous system,expressed in different tissues, mostly in intestine, placenta andlung. Post-translational modifications: Phosphorylated upon FLT3 and KIT signaling. Ligand-activatedALK induces tyrosine phosphorylation (By similarity).Dephosphorylated by PTPRJ at Tyr-205 (By similarity). Duallyphosphorylated on Thr-203 and Tyr-205, which activates the enzyme. Similarity: Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. Database links: UniProtKB/Swiss-Prot: P21708.5 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化丝裂原活化蛋白激酶3抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：**学 神经生物学 信号转导 干细胞 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid