立即早期癌基因BRLF1/鼻咽癌基因抗体
规格：1mg/1ml
英文名： BRLF1
别名： Replication and transcription activator; Rta; Immediate-early protein Rta; HHV4tp2_gp33; BRLF1 [Human herpesvirus 4 type 2].
分子量： 66.5kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human herpesvi
交叉反应：HHV4tp2
细胞定位：
立即早期癌基因BRLF1/鼻咽癌基因抗体产品介绍：background: Expression of the Epstein-Barr virus (EBV) immediate-early (IE) protein BRLF1 induces the lytic form of viral replication in most EBV-positive cell lines. BRLF1 is a transcriptional activator that binds directly to a GC-rich motif present in some EBV lytic gene promoters. However, BRLF1 activates transcription of the other IE protein, BZLF1, through an indirect mechanism which we previously showed to require activation of the stress mitogen-activated protein kinases. Here we demonstrate that BRLF1 activates phosphatidylinositol-3 (PI3) kinase signaling in host cells. We show that the specific PI3 kinase inhibitor, LY294002, completely 立即早期癌基因BRLF1/鼻咽癌基因抗体abrogates the ability of a BRLF1 adenovirus vector to induce the lytic form of EBV infection, while not affecting lytic infection induced by a BZLF1 adenovirus vector. Furthermore, we demonstrate that the requirement for PI3 kinase activation in BRLF1-induced transcriptional activation is promoter dependent. BRLF1 activation of the SM early promoter (which occurs through a direct binding立即早期癌基因BRLF1/鼻咽癌基因抗体 mechanism) does not require PI3 kinase activation, whereas activation of the IE BZLF1 and early BMRF1 promoters requires PI3 kinase activation. Thus, there are clearly two separate mechanisms by which BRLF1 induces transcriptional activation. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. BRLF1是EBV的立即早期基因。作为一种反式激活因子，它可以调节EBV早期／晚期基因的表达．并且还可能参与裂解期病毒基因组的复制。它的表达与EBV潜伏周期向裂解周期的转换密切相关。BRLF1的蛋因白产物Rta包含CTL识别的表位，可能在病毒裂解周期的早期成为**系统的作用位点。对它研究还可能为某些EBV相关肿瘤的筛查和**提供线索。
产立即早期癌基因BRLF1/鼻咽癌基因抗体品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **及病毒
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid