雌**反应锌指蛋白抗体
规格：1mg/1ml
英文名： TRIM25
别名： EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite motif containing 25; Tripartite motif containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147
分子量： 71kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TRIM25
交叉反应：Human, Mouse, Rat, Dog, Cow, Sheep,
细胞定位：细胞浆
雌**反应锌指蛋白抗体产品介绍：background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. Function: Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response 雌**反应锌指蛋白抗体to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Subunit: Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction. Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous.雌**反应锌指蛋白抗体 Post-translational modifications: Auto-ISGylated. Similarity: Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. Database links: UniProtKB/Swiss-Prot: Q14258.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
雌**反应锌指蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 发育生物学 染色质和核信号 锌指蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid