磷酸化p21激活激酶4/5/6抗体
规格：1mg/1ml
英文名： Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)
别名： PAK4 (phospho S474); p-PAK4 (phospho S474); Phospho-PAK4(Ser474); Phospho-PAK5(Ser602); Phospho-PAK6(Ser560); PAK4 + PAK5 + PAK6 (phospho S474 + S602 + S560); p21 activated kinase 4 ; p21(CDKN1A) acti
分子量： 64kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞浆
磷酸化p21激活激酶4/5/6抗体产品介绍：background: p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins. Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays 磷酸化p21激活激酶4/5/6抗体a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Subunit: Interacts with FGFR2 and GRB2. Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1. Subcellular Location: Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways. Tissue Specificity: Highest expression in prostate, testis and colon. Post-translational modifications: Autophosphorylated on serine residues when activated by CDC42/p21. Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity). Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. 磷酸化p21激活激酶4/5/6抗体STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. Gene ID: 10298 Database links: Entrez Gene: 10298 Human Entrez Gene: 70584 Mouse Entrez Gene: 292756 Rat Omim: 605451 Human SwissProt: O96013 Human SwissProt: Q8BTW9 Mouse Unigene: 20447 Human Unigene: 21876 Mouse Unigene: 18543 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 丝氨磷酸化p21激活激酶4/5/6抗体酸/苏氨酸蛋白激酶4 (PAK4)是新发现的一类蛋白因子，在细胞信号转导过程中起很重要的作用。Pak4常在人体的多种组织器官的肿瘤细胞中呈现过表达，他的丝氨酸位点很可能是Pak4酶活性区域中的自身磷酸化位点，丝氨酸到谷氨酸位点的突变体促使其获得了酶活性。在肿瘤遗传学、细胞癌变、细胞凋亡等一些重要的细胞生物学研究中，具有重要的意义。
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid