3磷酸肌醇依赖性蛋白激酶1抗体
规格：1mg/1ml
英文名： PDPK1
别名： 3 Phosphoinositide Dependent Protein Kinase 1; hPDK 1; hPDK 1; PDK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PDPK1_HUMAN.
分子量： 61kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PDK1
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
细胞定位：细胞浆 细胞膜
3磷酸肌醇依赖性蛋白激酶1抗体产品介绍：background: PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms. Function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. Subunit: Interacts with NPRL2. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to3磷酸肌醇依赖性蛋白激酶1抗体 its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. Tissue Specificity: Appears to be expressed ubiquitously. Post-translational modifications: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain. Gene ID: 5170 Database 3磷酸肌醇依赖性蛋白激酶1抗体links: Entrez Gene: 5170 Human Entrez Gene: 18607 Mouse Entrez Gene: 81745 Rat Omim: 605213 Human SwissProt: O15530 Human SwissProt: Q9Z2A0 Mouse SwissProt: O55173 Rat Unigene: 459691 Human Unigene: 10504 Mouse Unigene: 10905 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PDK1是蛋白激酶B(PKB)的上游激酶，通过与3,4,5三磷酸磷脂酰肌醇作用激活相邻的PKB分子。PDK1能够激活包含PKB在内的一系列的AGC激酶家族成员。从而调节细胞代谢，生长，扩散，生存，抗凋亡等诸多生理过程。
3磷酸肌醇依赖性蛋白激酶1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid