CD74抗体
规格：1mg/1ml
英文名： CD74
别名： CD 74; CD74; CD74 antigen; CD74 molecule; CLIP; DHLAG; Gamma chain of class II antigens; HG2A_HUMAN; HLA class II histocompatibility antigen gamma chain; HLA DR antigens associated invariant chain; HL
分子量： 34kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human CD74
交叉反应：Human, Mouse, Rat,
细胞定位：细胞膜
CD74抗体产品介绍：background: CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Function: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides toCD74抗体MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Subunit: Homotrimer. In the endoplasmic reticulum (ER) it forms a heterononameric MHC II-Ii complex: 3 MHC class II molecules (heterodimers of an alpha and a beta subunit) bind to the CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system, the CD74 component undergoes sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide) attached to the MHC class II molecule (alpha-beta-CLIP complex). This processed complex interacts with HLA_DM and HLA_DO heterodimers in order to release CLIP and facilitate the binding of antigenic peptides to the MHC class II molecules. Subcellular Location: Cell membrane; Single-pass type II membrane protein (Potential). Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Note=Transits through aCD74抗体 number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. Post-translational modifications: N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. DISEASE: Note=A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74-ROS1 chimeric protein. Similarity: Contains 1 thyroglobulin type-1 domain. Gene ID: 972 Database links: Entrez Gene: 972 Human Entrez Gene: 16149 Mouse Omim: 142790 Human SwissProt: P04233 Human SwissProt: P04441 Mouse Unigene: 436568 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD74表达于所有B细胞和某些T细胞亚类（MHC II阳性），具有几种异构体形式（33，35和41kDa），是HLA-DR的恒定链。该抗体主要用于标记**中心及外套B细胞（80%）和R-S细胞（70%）以及少数T细胞（20%），常与其它B细胞标记如CD20或CD45RA联合检测，用于B细胞**瘤的辅助诊断。
CD74抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 细胞表面分子 细胞类型标志物 **细胞 t-**细胞 b-**细胞
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid