乙酰胆碱酶抗体
规格：1mg/1ml
英文名： ACHE
别名： Acetylcholinesterase; Acetylcholine acetylhydrolase; Acetylcholinesterase YT blood group; ACHE; ACHE protein; Apoptosis related acetylcholinesterase; ARACHE; N ACHE; N-ACHE; YT; Yt blood group; ACES_H
分子量： 68kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human AchE
交叉反应：Human, Mouse, Rat, Dog, Cow, Horse,
细胞定位：
乙酰胆碱酶抗体产品介绍：background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Subunit: Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them 乙酰胆碱酶抗体to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric. Subcellular Location: Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein. Isoform T: Nucleus. Note=Only observed in apoptotic nuclei. Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Tissue Specificity: Isoform H is highly expressed in erythrocytes. Similarity: Belongs to 乙酰胆碱酶抗体the type-B carboxylesterase/lipase family. Gene ID: 43 Database links: Entrez Gene: 540446 Cow Entrez Gene: 43 Human Entrez Gene: 11423 Mouse Entrez Gene: 83817 Rat Omim: 100740 Human SwissProt: O62763 Cat SwissProt: P23795 Cow SwissProt: P22303 Human SwissProt: P21836 Mouse SwissProt: P37136 Rat Unigene: 154495 Human Unigene: 255464 Mouse Unigene: 105879 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
乙酰胆碱酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：神经生物学 信号转导
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid