染色质组装因子1P60抗体
规格：1mg/1ml
英文名： p60 CAF1
别名： CAF 1 subunit B; CAF 1A; CAF I 60 kDa subunit; CAF IP60; CAF-1; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CAF1; CAF1A; CAF1B_HUMAN; CAF1P60; CHAF 1B; CHAF1B; Chromatin assembly factor 1 subuni
分子量： 61kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human p60 CAF1
交叉反应：Human, Rat,
细胞定位：细胞核 细胞浆
染色质组装因子1P60抗体产品介绍：background: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008] Function: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin 染色质组装因子1P60抗体precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Subcellular Location: Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase. Post-translational modifications: Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in 染色质组装因子1P60抗体G1, S or G2 phases. Similarity: Belongs to the WD repeat HIR1 family. Contains 7 WD repeats. Database links: Entrez Gene: 8208 Human Entrez Gene: 288242 Rat Omim: 601245 Human SwissProt: Q13112 Human Unigene: 75238 Human Unigene: 159953 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
染色质组装因子1P60抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid