微小染色体维持缺陷蛋白2抗体
规格：1mg/1ml
英文名： MCM2
别名： BM28; CCNL 1; CCNL1; CDC like 1; CDC like-1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; cyclin like-1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2
分子量： 102kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from mouse MCM2
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
细胞定位：细胞核
微小染色体维持缺陷蛋白2抗体产品介绍：background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring 微小染色体维持缺陷蛋白2抗体subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with KAT7 and DBF4. May interact with MCM10. Subcellular Location: Nucleus (Probable). Post-translational modifications: Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the微小染色体维持缺陷蛋白2抗体 CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. Similarity: Belongs to the MCM family. Contains 1 MCM domain. Gene ID: 17216 Database links: Entrez Gene: 4171 Human Entrez Gene: 17216 Mouse Entrez Gene: 312538 Rat Omim: 116945 Human SwissProt: P49736 Human SwissProt: P97310 Mouse Unigene: 477481 Human Unigene: 16711 Mouse Unigene: 2715 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 微小染色体维持缺陷蛋白2又称微微小染色体维持缺陷蛋白2抗体型染色体维持蛋白2或丝裂蛋白，MCM-2是细胞周期阶段的特异性标志物,在进入细胞周期的细胞中均有表达，在细胞的增值、分化中起到一定的作用。
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：染色质和核信号 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid