Ras蛋白特异鸟嘌呤核苷酸释放因子2抗体
规格：1mg/1ml
英文名： RASGRP2
别名： Calcium and DAG-regulated; Calcium and DAG-regulated guanine nucleotide exchange factor I; Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25 like protein; C
分子量： 69kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RASGRP2
交叉反应：Human, Mouse, Rat, Cow,
细胞定位：细胞浆
Ras蛋白特异鸟嘌呤核苷酸释放因子2抗体产品介绍：background: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Function: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. Subcellular Location: Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > Ras蛋白特异鸟嘌呤核苷酸释放因子2抗体synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons. Tissue Specificity: Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney. Post-translational modifications: Isoform 2 is palmitoylated and myristoylated while isoform 1. Similarity: Belongs to the RASGRPRas蛋白特异鸟嘌呤核苷酸释放因子2抗体 family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain. Gene ID: 10235 Database links: Entrez Gene: 10235 Human Entrez Gene: 512056 Cow Entrez Gene: 19395 Mouse Entrez Gene: 361714 Rat Omim: 605577 Human SwissProt: A6N9I4 Cow SwissProt: Q7LDG7 Human SwissProt: Q9QUG9 Mouse SwissProt: P0C643 Rat Unigene: 99491 Human Unigene: 77017 Mouse Unigene: 38473 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Ras蛋白特异鸟嘌呤核苷酸释放因子2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 结合蛋白 G蛋白偶联受体 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid