卵磷酯胆固醇?；泼缚固?/strong>
规格：1mg/1ml
英文名： LCAT
别名： LCAT; LCAT_HUMAN; Lecithin cholesterol acyltransferase; Lecithin-cholesterol acyltransferase; Phosphatidylcholine sterol acyltransferase; Phosphatidylcholine-sterol acyltransferase; Phospholipid chole
分子量： 47kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human LCAT
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
细胞定位：分泌型蛋白
卵磷酯胆固醇?；泼缚固?/strong>产品介绍：background: This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008] Function: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Subcellular Location: Secreted. Note=Secreted into blood plasma卵磷酯胆固醇?；泼缚固?/strong> Produced in astrocytes and secreted into cerebral spinal fluid (CSF). Tissue Specificity: Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. Expressed in Hep-G2 cell line. Post-translational modifications: O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures. DISEASE: Lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]: A disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: complete LCAT deficiency and fish-eye disease. LCATD is generally referred to the complete form which is associated with absence of both alpha and beta LCAT activities resulting in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure. Note=The disease is caused by mutations affecting the gene represented in this entry.卵磷酯胆固醇?；泼缚固?/strong> Fish-eye disease (FED) [MIM:136120]: A disorder of lipoprotein metabolism due to partial lecithin-cholesterol acyltransferase deficiency that affects only alpha-LCAT activity. FED is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye'). Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the AB hydrolase superfamily. Lipase family. Gene ID: 3931 Database links: Entrez Gene: 3931 Human Entrez Gene: 16816 Mouse Entrez Gene: 24530 Rat Omim: 606967 Human SwissProt: P04180 Human 卵磷酯胆固醇?；泼缚固?/strong>SwissProt: P16301 Mouse SwissProt: P18424 Rat Unigene: 387239 Human Unigene: 1593 Mouse Unigene: 10481 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. LCAT是参与体内脂质代谢的主要成份之一，是一种在高密度脂蛋白(HDL)代谢和动脉粥样硬化(AS)发展中的关键酶。LCAT需要经载脂蛋白ApoAI、载脂蛋白D(ApoD)作为辅助因子并经ApoE活化来发挥作用。
产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 心血管 **学 神经生物学 信号转导 激酶和磷酸酶 新陈代谢
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid