乳腺癌相关蛋白SGA 1M抗体
规格：1mg/1ml
英文名： NFIP1
别名： 0610010M22Rik; Breast cancer associated protein SGA 1M; Breast cancer-associated protein SGA-1M; MGC10924; N4WBP5; Ndfip1; Nedd4 family interacting protein 1; NEDD4 family-interacting protein 1; NEDD4
分子量： 25kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NFIP1
交叉反应：Human, Mouse, Rat,
细胞定位：
乳腺癌相关蛋白SGA 1M抗体产品介绍：background: The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008] Function: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. Subunit: Forms heterodimers with NDFIP2. Interacts with several E3 ubiquitin-protein ligases, including NEDD4乳腺癌相关蛋白SGA 1M抗体, NEDD4L and WWP2. Interacts with ITCH, U2SURP and WWP2 By similarity. The interaction with NEDD4, NEDD4L and ITCH leads to relocalization of these proteins to exosomes and eventually to exosomal secretion. Interacts with SLC11A2/DMT1. Interacts with PTEN. May interact with phosphorylated EGFR. Subcellular Location: Endosome membrane. Golgi apparatus membrane. Secreted. Detected in exosomes and secreted via the exosomal pathway. Tissue Specificity: Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver乳腺癌相关蛋白SGA 1M抗体, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung. Post-translational modifications: Ubiquitinated by NEDD4 and ITCH; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation. Undergoes transient tyrosine phosphorylation following EGF stimulation, most probably by catalyzed by SRC. Phosphorylation SRC is enhanced in the presence of NDFIP2 which may act as a scaffold to recruit SRC to NDFIP1. Database links: Entrez Gene: 80762 Human Entrez Gene: 65113 Mouse Entrez Gene: 291609 Rat Omim: 612050 Human SwissProt: Q9BT67 Human SwissProt: Q8R0W6 Mouse SwissProt: Q5U2S1 Rat Unigene: 653510 Human Unigene: 9788 Human Unigene: 102496 Mouse Unigene: 59254 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
乳腺癌相关蛋白SGA 1M抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 表观遗传学 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid