异型组蛋白H2A.1抗体
规格：1mg/1ml
英文名： macro H2A.1
别名： Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2
分子量： 39kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human macro H2
交叉反应：Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep, Guinea Pig,
细胞定位：细胞核
异型组蛋白H2A.1抗体产品介绍：background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair,异型组蛋白H2A.1抗体 DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in异型组蛋白H2A.1抗体 ADP-ribose-mediated chromatin modulation. Subcellular Location: Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. Tissue Specificity: Ubiquitous. Post-translational modifications: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin. Similarity: Contains 1 histone H2A domain. Contains 1 Macro domain. Gene ID: 9555 Database links: Entrez Gene: 9555 Human Entrez Gene: 26914 Mouse Entrez Gene: 29384 Rat Omim: 610054 Human SwissProt: O75367 Human SwissProt: Q9QZQ8 Mouse SwissProt: Q02874 Rat Unigene: 420272 Human Unigene: 599225 Human Unigene: 283802 Mouse Unigene: 478369 Mouse Unigene: 11098 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
异型组蛋白H2A.1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid