DNA拓扑异构酶2结合蛋白1抗体
规格：1mg/1ml
英文名： TopBP1
别名： DNA topoisomerase 2 binding protein 1; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II binding protein 1; DNA topoisomerase II binding protein; DNA topoisomerase II-beta-binding protein 1;
分子量： 171kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TopBP1
交叉反应：Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
细胞定位：细胞浆 细胞膜
DNA拓扑异构酶2结合蛋白1抗体产品介绍：background: Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Subcellular Location: Nucleus. Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y DNA拓扑异构酶2结合蛋白1抗体chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Tissue Specificity: Highly expressed in heart,DNA拓扑异构酶2结合蛋白1抗体 brain, placenta, lung and kidney. Post-translational modifications: Phosphorylated on serine and threonine residues in response to X-ray irradiation. Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Similarity: Contains 8 BRCT domains. Gene ID: 11073 Database links: Entrez Gene: 11073 Human Entrez Gene: 235559 Mouse Omim: 607760 Human SwissProt: Q92547 Human SwissProt: Q6ZQF0 Mouse Unigene: 593379 Human Unigene: 259893 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
DNA拓扑异构酶2结合蛋白1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid